Endotoxin Removal from Recombinant Human-like Collagen Preparations by Triton X-114 Two-phase Extraction
نویسندگان
چکیده
منابع مشابه
Removal of endotoxin from protein solutions by phase separation using Triton X-114.
Endotoxin contamination of protein solutions was reduced by a phase separation technique using the detergent, Triton X-114. Protein solutions containing endotoxin were treated with Triton X-114 on ice. The solution was then warmed to 37 degrees C, whereupon two phases formed. The Triton X-114 phase, containing the endotoxin, was precipitated by centrifugation. The first cycle of phase separatio...
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ژورنال
عنوان ژورنال: Biotechnology(Faisalabad)
سال: 2013
ISSN: 1682-296X
DOI: 10.3923/biotech.2013.135.139